The information relevant to the vascular density is also important, because angiogenesis is an essential mechanism for post-ischemic recovery. The perfusion rate estimated from the time-series ICG images is not enough for the evaluation of hindlimb ischemia. In vivo time-series indocyanine green (ICG) fluorescence imaging can noninvasively monitor blood flow and has a deep tissue penetration. Quantitative evaluation of the vascular perfusion in the ischemic limb contributes to diagnosis of PAD and preclinical development of new drug. Peripheral arterial disease (PAD) can further cause lower limb ischemia. Guang, Huizhi Cai, Chuangjian Zuo, Simin Cai, Wenjuan Zhang, Jiulou Luo, Jianwen Multiparametric evaluation of hindlimb ischemia using time-series indocyanine green fluorescence imaging.
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